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Beta-Actin Antibody(50ul)

Beta-Actin Antibody(50ul)

型    号: abs100041
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abs100041Beta-Actin Antibody(50ul)的详细资料:

概述
描述

Mouse Monoclonal Antibody (Mab)

别名Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB
克隆类别Monoclonal
克隆号137CT26.1.1
同种亚型IgG1,Igk
反应种属 H, M, R
预测反应种属 X, B, C, Pr, P, R
来源宿主Mouse
应用IHC-P, IF, WB
形态Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediay, followed by dialysis against PBS.
使用方法WB~~1:1000
IHC-P~~1:25
IF~~1:10~50
性能
生产说明This ACTB Monoclonal antibody is generated from mouse immunized with ACTB recombinant protein.
抗原来源HUMAN
背景This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
保存方法Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
研究领域Crown Antibodies;Cancer;Signal Transduction;Loading Control
分子量41.7 kDa
参考文献
Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 Apr 8. PMID 20381070. Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor alpha in human breast cancer cell nuclei. Ambrosino C, et al. Mol Cell Proteomics, 2010 Jun. PMID 20308691. Contribution of rearranged actin structures to the spread of Ectromelia virus infection in vitro. Boratynska A, et al. Acta Virol, 2010. PMID 20201613. Molecular mechanisms underlying nucleocytoplasmic shuttling of actinin-4. Kumeta M, et al. J Cell Sci, 2010 Apr 1. PMID 20197409. Tyrosine phosphorylation of cofilin at Y68 by v-Src leads to its degradation through ubiquitin-proteasome pathway. Yoo Y, et al. Oncogene, 2010 Jan 14. PMID 19802004.

实验结果图

All lanes : Anti-ACTB Antibody at 1:1000 dilutionLane 1: A431 whole cell lysaane 2: C2C12 whole cell lysaane 3: C6 whole cell lysaane 4: Hela whole cell lysaane 5: MCF-7 whole cell lysaysates/proteins at 20 μg per lane. SecondaryGoat Anti-mouse IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-ACTB Antibody at 1:1000 dilutionLane 1: A431 whole cell lysaane 2: C2C12 whole cell lysaane 3: C6 whole cell lysaane 4: Hela whole cell lysaane 5: MCF-7 whole cell lysaysates/proteins at 20 μg per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin  Antibody(Cat#abs100041). abs100041 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin Antibody(Cat#abs100041). abs100041 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin  Antibody(Cat#abs100041). abs100041 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin Antibody(Cat#abs100041). abs100041 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of anti-ACTB Antibody (Cat. #abs100041) in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35μg/lane). ACTB (arrow) was detected using the purified Mab.

Western blot analysis of anti-ACTB Antibody (Cat. #abs100041) in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35μg/lane). ACTB (arrow) was detected using the purified Mab.

Confocal immunofluorescent analysis of ACTB Antibody (Cat#abs100041) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

Confocal immunofluorescent analysis of ACTB Antibody (Cat#abs100041) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).


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