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Beta-Actin Antibody(50ul)

Beta-Actin Antibody(50ul)

型    号: abs100006
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abs100006Beta-Actin Antibody(50ul)的详细资料:

概述
描述Purified Mouse Monoclonal Antibody (Mab)
别名Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB
克隆类别Monoclonal
克隆号8H10D10
反应种属 H, M, R
来源宿主Mouse
应用WB, IHC-P
形态Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediay, followed by dialysis against PBS.
使用方法WB~~1:1000
IHC-P~~1:10~50
性能
生产说明ACTB recombinant protein is used to produce this monoclonal antibody.
抗原来源HUMAN
背景This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
保存方法Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
研究领域Crown Antibodies;Cancer;Signal Transduction;Loading Control
分子量41.7 kDa
参考文献
Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 Apr 8. PMID 20381070. Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor alpha in human breast cancer cell nuclei. Ambrosino C, et al. Mol Cell Proteomics, 2010 Jun. PMID 20308691. Contribution of rearranged actin structures to the spread of Ectromelia virus infection in vitro. Boratynska A, et al. Acta Virol, 2010. PMID 20201613. Molecular mechanisms underlying nucleocytoplasmic shuttling of actinin-4. Kumeta M, et al. J Cell Sci, 2010 Apr 1. PMID 20197409. Tyrosine phosphorylation of cofilin at Y68 by v-Src leads to its degradation through ubiquitin-proteasome pathway. Yoo Y, et al. Oncogene, 2010 Jan 14. PMID 19802004.

实验结果图

Western blot analysis of lysates from HepG2, HL-60 cell line (from left to right),  using Beta-actin Antibody(Cat.  #abs100006).  abs100006 was diluted at 1:1000 at each lane.  A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20μg per lane.

Western blot analysis of lysates from HepG2, HL-60 cell line (from left to right), using Beta-actin Antibody(Cat. #abs100006). abs100006 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20μg per lane.

Immunohistochemical analysis of paraffin-embedded H. spleen section using Beta-actin Antibody(Cat#abs100006). abs100006 was diluted at 1:25 dilution.  A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody,  followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. spleen section using Beta-actin Antibody(Cat#abs100006). abs100006 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from HepG2, HL-60, mouse NIH/3T3 cell line, mouse cerebellum and rat stomach tissue lysate, CHO, COS-7 cell line lysate(from left to right), using Beta-actin Antibody(Cat.  #abs100006). abs100006 was diluted at 1:1000 at each lane.  A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

Western blot analysis of lysates from HepG2, HL-60, mouse NIH/3T3 cell line, mouse cerebellum and rat stomach tissue lysate, CHO, COS-7 cell line lysate(from left to right), using Beta-actin Antibody(Cat. #abs100006). abs100006 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

Immunohistochemical analysis of paraffin-embedded H. spleen section using Beta-actin Antibody(Cat#abs100006). abs100006 was diluted at 1:25 dilution.  A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody,  followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. spleen section using Beta-actin Antibody(Cat#abs100006). abs100006 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.


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